Human IFN gamma ELISpot Kit - Detachable Strips (A334654)

Human IFN gamma ELISpot Kit - Detachable Strips

Human IFN gamma ELISpot Kit (Detachable Strips) is a ready-to-use ELISpot assay for the in vitro detection and quantification of IFN gamma-secreting human PBMCs at the single-cell level.

sandwich ELISA

The Human IFN gamma ELISpot Kit (A334653/A334654) employs the double-antibody sandwich ELISpot technique for the detection and quantification of IFN gamma-secreting cells at the single-cell level. A capture antibody specific for human IFN gamma is pre-coated onto a PVDF 96-well microplate (or detachable strip format). Test cells, such as peripheral blood mononuclear cells (PBMCs), are added into the wells and, upon stimulation, secrete IFN gamma that is captured by the immobilized antibody. A biotinylated detection antibody specific for IFN gamma is then added, which binds to the captured cytokine, followed by Streptavidin-HRP, which binds the biotinylated antibody. After incubation, unbound detection antibody and Streptavidin-HRP are removed by washing, and an AEC substrate solution is added. Spots develop at the sites of cytokine secretion in proportion to the number of IFN gamma-producing cells. The color development can be quantified using an ELISpot reader, providing the frequency of cytokine-secreting cells in the sample. This kit is ready-to-use, including pre-coated plates/strips, detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, and washing buffer.

Biotinylated Antibody 100µl 1 vial; Streptavidin-HRP 100µl 1 vial; Cell Stimulant 50 T 1 bottle; Dilution Buffer R (1×) 10mL 2 bottles; Washing Buffer (50×) 15mL 1 bottle; AEC Dilution 10mL 1 bottle; AEC Solution I (20×) 500µl 1 vial; AEC Solution II (20×) 500µl 1 vial; AEC Solution III (200×) 50µl 1 vial; Pre-coated PVDF Plate/1 plate with detachable strips.

Colorimetric

ELISpot Plate Reader or Image Capture and Quantification. Spots on the membrane are counted, with each spot corresponding to one secreting cell, providing a quantitative measure of cellular activity.

Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.

Assay time: 3 hours. Sample incubation time: 16-24 hours.

This ELISpot Kit specifically targets Human IFN gamma.

Human

Intra-assay CV: < 15%, Inter-assay CV: < 20%

Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.

IFN-gamma, IFNG, Immune interferon, Interferon gamma

Information online is representative. Always refer to the Product Manual inside the kit.