Human IFN gamma + Granzyme B Dual ELISpot Matched Antibody Pair Kit (A341792)

Human IFN gamma + Granzyme B Dual ELISpot Matched Antibody Pair Kit

IFN gamma + Granzyme B complete ELISpot kit includes pre-validated capture and detection antibodies for both targeted cytokines, together with streptavidin-alkaline phosphatase conjugate, anti-FITC HRP-conjugated monoclonal antibody, BSA, BCIP/NBT and AEC substrates, as well as blocking reagent. The kit is designed for sensitive, single-cell ELISpot detection of IFN gamma + Granzyme B secretion in PBMCs.

Human IFN gamma + Granzyme B Dual ELISpot Matched Antibody Pair Kit (A341792) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IFN gamma + Granzyme B in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IFN gamma + Granzyme B . Standards and samples are added to the wells, allowing IFN gamma + Granzyme B present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IFN gamma + Granzyme B is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IFN gamma + Granzyme B in the sample. The concentration of IFN gamma + Granzyme B is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Colorimetric

3h 30m after cell stimulation

This product recognizes natural human IFN-g and Granzyme B.

Human

This product shows no cross reactivity with other human cytokines. Cross reactivity with simian IFN gamma.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.