Human IgA2 ELISA Kit is a 3 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IgA2 in serum, plasma, tissue homogenates, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human IgA2 ELISA Kit (A76753) is a 3 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IgA2 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for IgA2. Standards and samples are added to the wells, where IgA2 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for IgA2 is added to the wells. The detection antibody binds the captured IgA2 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IgA2 captured in each well. The concentration of IgA2 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.375 ng/mL
Product Range
0.625-40 ng/mL
Assay Time
3 hours
Reactivity
Human
Cross Reactivity
Specific for IgA2 with no reported cross-reactivity with other analogs or variants.
Recovery
Sample Type
n
Range
Average
Serum
10
89% - 97%
93%
EDTA Plasma
10
87% - 101%
96%
Heparin Plasma
10
85% - 100%
91%
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
89-100%
89-104%
93-105%
EDTA Plasma
10
84-95%
85-101%
82-93%
Heparin Plasma
10
83-99%
80-97%
80-95%
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Synonyms
A2m marker, Ig alpha 2 chain C region, IgA constant heavy chain 2, IGHA 2, IGHA2, IGHA2 protein, Immunoglobulin A2, Immunoglobulin Am2, Immunoglobulin heavy constant alpha 2, immunoglobulin heavy constant alpha 2 (A2m marker)
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Human IgA2 ELISA Kit (A76753) across serial sample dilutions. Linearity was evaluated using Serum, EDTA Plasma, and Heparin Plasma samples serially diluted from 1:2 to 1:8. Recovery (%) was calculated relative to the expected Human IgA2 concentration at each dilution. Data are presented as mean recovery values from 10 replicates (n = 10).
Publishing research using Human IgA2 ELISA Kit (A76753)? Please let us know so that we can list the citation on this page.
