Human IgA2 ELISA Kit (A76753)

Human IgA2 ELISA Kit

Human IgA2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IgA2 in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Human IgA2 ELISA Kit (A76753) employs HRP antibody sandwich ELISA for the quantitative measurement of human IgA2 in serum, plasma, tissue homogenates, and other biological fluids. Capture antibody specific for IgA2 is pre-coated on a 96-well microtiter plate. Samples and standards are added, and IgA2 binds to the immobilized antibody. Detection is performed using HRP-conjugated detection antibody, which binds to a different epitope on the captured target. Following another wash, TMB substrate solution produces a blue-coloured product that changes to yellow after addition of acidic stop solution. The intensity of yellow is proportional to the amount of IgA2 captured, and the concentration is determined by reading O.D. at 450nm.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

0.375 ng/ml

0.625-40 ng/ml

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

A2m marker, Ig alpha 2 chain C region, IgA constant heavy chain 2, IGHA 2, IGHA2, IGHA2 protein, Immunoglobulin A2, Immunoglobulin Am2, Immunoglobulin heavy constant alpha 2, immunoglobulin heavy constant alpha 2 (A2m marker)

Information online is representative. Always refer to the Product Manual inside the kit.