Human IL-17F ELISpot Kit (A341820)

Human IL-17F ELISpot Kit

Human IL-17F ELISpot kit includes pre-coated PVDF plates, detection antibody, alkaline phosphatase conjugate, BSA, and BCIP/NBT ready-to-use substrate buffer. The kit is designed for sensitive, single-cell ELISpot detection of Human IL-17F secretion in PBMCs.

Human IL-17F ELISpot Kit (A341820) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IL-17F in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IL-17F. Standards and samples are added to the wells, allowing IL-17F present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IL-17F is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IL-17F in the sample. The concentration of IL-17F is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

3h after cell stimulation

This product recognizesnatural human IL-17F.

Human

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

Cytokine ML-1, IL17F, Interleukin-17F

Information online is representative. Always refer to the Product Manual inside the kit.