Human IL-2 ELISA Kit (High Sensitivty) (A334776)

Human IL-2 ELISA Kit (High Sensitivty)

Human IL-2 ELISA Kit (High Sensitivty) is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-2 in serum and plasma.

Sandwich (quantitative)

Human IL-2 ELISA Kit (High Sensitivty) (A334776) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human IL-2 in serum and plasma. A 96-well microtiter plate is provided pre-coated with an antibody specific for IL-2. Standards and test samples are added to the wells, allowing IL-2 to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of IL-2 captured in each well. The concentration of IL-2 is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 30 mL Assay Diluent #11E4, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well A-Microplate (12 × 8 well strips), and 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum and plasma.

3.13 pg/ml - 200 pg/ml

1 hour

Human

Serum: 111%; Plasma: 92%

Intra-assay:4.4%, Inter-assay: 6.2%

The immunogens for the antibodies in this kit are recombinant IL-2.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

This ELISA kit uses recombinant rabbit monoclonal antibodies.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

IL2, Interleukin-2, T-cell growth factor, TCGF

Information online is representative. Always refer to the Product Manual inside the kit.