Recombinant Human IL-5 ELISA Kit (A334784)

Human IL-5 ELISA Kit

Human IL-5 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-5 in serum, plasma, and cell culture supernatant.

Sandwich (quantitative)

Human IL-5 ELISA Kit (A334784) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human IL-5 in serum, plasma, and cell culture supernatant. An antibody specific for IL-5 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-5 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-5 Antibody, which binds the captured IL-5 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-5 captured in each well. The concentration of IL-5 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 30 mL Assay Diluent (1), 40 mL Diluent (3), 30 mL Assay Diluent (1S5), 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well H-Microplate 12 x 8 well strips, 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

1.1 pg/ml

3.9 pg/ml - 250 pg/ml

1 hour

Human

Cell culture supernatant: 85%; Serum: 102%; Plasma: 97%

Intra-assay: 4.7%, Inter-assay: 6.4%

The immunogens for the antibodies in this kit are recombinant IL-5.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

B-cell differentiation factor I, Eosinophil differentiation factor, IL5, Interleukin-5, T-cell replacing factor, TRF

Information online is representative. Always refer to the Product Manual inside the kit.