Human IL-6 ELISpot kit includes pre-coated PVDF plates, detection antibody, alkaline phosphatase conjugate, BSA, and BCIP/NBT ready-to-use substrate buffer. The kit is designed for sensitive, single-cell ELISpot detection of Human IL-6 secretion in PBMCs.
Principle of Assay
Human IL-6 ELISpot Kit (A341808) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IL-6 in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IL-6. Standards and samples are added to the wells, allowing IL-6 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IL-6 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IL-6 in the sample. The concentration of IL-6 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
Platform
Pre-coated Microplate (1 x 96)
Detection Type
Colorimetric
Sample Type
Peripheral blood mononuclear cells (PBMCs).
Assay Time
3h after cell stimulation
Specificity
This product recognizes natural human IL-6.
Reactivity
Human
Cross Reactivity
This product shows no cross reactivity with other human cytokines. No interference with soluble receptor gp80.
General Notes
For bulk or custom IVD raw material requests, contact us directly. IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.
