Human Interleukin-17A ELISA Kit (A33035)

Human Interleukin-17A ELISA Kit

Sandwich (quantitative)

This IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for IL-17A was added and incubated. IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-17A and other components of the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

In order to measure the concentration of IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-17A concentration (pg/mL). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

< 5 pg/ml

5-1000 pg/ml

3h 30m

This sandwich ELISA recognises both natural and recombinant human IL-17A.

Human

This assay has shown no cross-reactivity with other cytokines tested.

Store at 2-8°C.

CTLA 8, CTLA-8, CTLA8, Cytotoxic T lymphocyte associated antigen 8, Cytotoxic T lymphocyte associated protein 8, Cytotoxic T lymphocyte associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL 17, IL 17A, IL-17, IL-17A, IL17, Il17a, IL17_HUMAN, Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8), Interleukin 17A, Interleukin-17A, Interleukin17, OTTHUMP00000016597, OTTMUSP00000046003

Information online is representative. Always refer to the Product Manual inside the kit.