Human ITIH4 ELISA Kit is an indirect elisa Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human ITIH4 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Assay Type
Indirect (quantitative)
Principle of Assay
Human ITIH4 ELISA Kit (A335043) is an Indirect ELISA for the quantitative measurement of human ITIH4 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The 96-well microtiter plate has been pre-coated with ITIH4 antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of ITIH4 sample captured on the plate. The concentration of ITIH4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Sensitivity
0.469 ng/ml
Product Range
0.781-50 ng/ml
Assay Time
3 hours
Reactivity
Human
Recovery
Sample Type
n
Range
Average
Serum
10
90-103
92
EDTA plasma
10
86-105
96
Heparin plasma
10
85-101
91
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
89-100%
81-86%
81-93%
EDTA plasma
10
84-98%
90-105%
89-104%
Heparin plasma
10
85-103%
83-92%
91-100%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
Gp120, IHRP, Inter-alpha-inhibitor heavy chain 4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, Inter-alpha-trypsin inhibitor heavy chain H4, ITI heavy chain H4, ITI-HC4, ITIHL1, PK-120, PK120, Plasma kallikrein sensitive glycoprotein 120
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
