Human Lipocalin-2 ELISA Kit (A1481)

Human Lipocalin-2 ELISA Kit

Human Lipocalin-2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Lipocalin-2 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Lipocalin-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Lipocalin-2 in the samples. A monoclonal antibody specific for human Lipocalin-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Lipocalin-2, and Lipocalin-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Lipocalin-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.42 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human Lipocalin-2.

Human

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Neutrophil gelatinase-associated lipocalin, NGAL, Oncogene 24p3, p25, Siderocalin

Information online is representative. Always refer to the Product Manual inside the kit.