Human Lysozyme ELISA Kit (A87375)

Human Lysozyme ELISA Kit

Human Lysozyme ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Lysozyme in serum, plasma, tissue homogenates, and other biological fluids.

Competitive (quantitative)

Human Lysozyme ELISA Kit (A87375) is a Competitive ELISA for the quantitative measurement of human Lysozyme in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Lysozyme has been pre-coated onto a 96-well microtiter plate. During the incubation, Lysozyme in the sample or standard competes with a fixed amount of Biotinylated-Lysozyme. Following incubation, Biotinylated-Lysozyme and free Lysozyme in the sample or standard are washed from the plate. HRP-Streptavidin (SABC) is then added to each well and incubated. Unbound SABC is washed away with wash buffer. TMB substrate solution is added and after incubation the enzyme-substrate reaction is terminated by the addition of sulfuric acid stop solution. The colour change is measured spectrophotometrically at a wavelength of 450nm and the concentration of Lysozyme in the samples is then determined by comparing the O.D. 450nm of the samples to the standard curve. O.D. 450nm is inversely proportional to the concentration of Lysozyme in the sample.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Serum, plasma, tissue homogenates, and other biological fluids.

5.625 ng/ml

9.375-600 ng/ml

2 hours

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

1,4-beta-N-acetylmuramidase C, Lysozyme C, LYZ, LZM

Information online is representative. Always refer to the Product Manual inside the kit.