Human M-CSF ELISA Kit (A1483)

Human M-CSF ELISA Kit

Human M-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human M-CSF in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human M-CSF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human M-CSF in the samples. A monoclonal antibody specific for human M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and M-CSF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for M-CSF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

Colorimetric

0.19 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human M-CSF.

Human

This ELISA kit has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CSF-1, CSF1, Lanimostim, Macrophage colony-stimulating factor 1, MCSF, PG-M-CSF, Proteoglycan macrophage colony-stimulating factor

Information online is representative. Always refer to the Product Manual inside the kit.