Human MCP 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MCP 1 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human MCP 1 ELISA Kit (A335009) is based on Double Antigen Sandwich ELISA technology for the quantitative measurement of human MCP 1 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The antigen has been pre-coated onto a 96-well microplate, and antibodies present in the sample bind to the immobilized antigen. A Biotinylated antigen is then added to detect the captured antibodies. The samples and standards are added to the wells and antibodies in each sample form a sandwich with the immobilized and biotinylated antigens. After washing with wash buffer to remove unbound components, HRP-Streptavidin Conjugate is added and incubated. After another wash, TMB substrate solution is used to visualize the HRP enzymatic reaction, producing a blue-colored product that changes to yellow after adding acidic stop solution. The intensity of the yellow color is proportional to the amount of antibodies captured on the plate. The concentration of antibodies can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Sensitivity
0.469 ng/ml
Product Range
0.781-50 ng/ml
Assay Time
3 hours
Reactivity
Human
Recovery
Sample Type
n
Range
Average
Serum
10
86-105
93
EDTA plasma
10
89-105
99
Heparin plasma
10
87-104
94
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
85-100%
83-101%
85-99%
EDTA plasma
10
90-104%
86-99%
82-92%
Heparin plasma
10
85-88%
86-97%
81-100%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
C-C motif chemokine 2, CCL2, HC11, MCAF, MCP-1, MCP1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
