Human MCP 1 ELISA Kit (A341779)

Human MCP 1 ELISA Kit

Human MCP 1 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human MCP 1 in biological samples.

Sandwich (quantitative)

Human MCP 1 ELISA Kit (A341779) is a sandwich ELISA designed for the sensitive and quantitative measurement of human MCP 1 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for MCP 1. Standards and samples are added to the wells, allowing MCP 1 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for MCP 1 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of MCP 1 in the sample. The concentration of MCP 1 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

5.8pg/ml

31.25-1000pg/ml

2h 15m

This product recognizesboth natural and recombinant human MCP-1.

Human

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

C-C motif chemokine 2, CCL2, HC11, MCAF, MCP-1, MCP1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Information online is representative. Always refer to the Product Manual inside the kit.