Human MDC ELISA Kit (A654)

Human MDC ELISA Kit

Sandwich (quantitative)

This MDC enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-MDC Antibody. Standards or samples are then added to the microtire plate wells and MDC, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of MDC present in the sample, Anti-MDC Antibody (Biotin) is added to each well to "sandwich" the MDC immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MDC will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 1 pg/ml

31.2-2000 pg/ml

Human

Store at 2-8°C for 4 months or at -20°C for 8 months.

C-C motif chemokine 22, CC chemokine STCP-1, CCL22, Macrophage-derived chemokine, MDC(1-69, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

Information online is representative. Always refer to the Product Manual inside the kit.