Human MIF ELISA Kit (A1485)

Human MIF ELISA Kit

Sandwich (quantitative)

Human MIF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MIF in the samples. A monoclonal antibody specific for human MIF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for MIF, and MIF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MIF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human MIF.

Human

This assay has shown no significant cross-reactivity.

105% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

EC:5.3.3.12, GIF, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase

Information online is representative. Always refer to the Product Manual inside the kit.