Principle of Assay
Human MMP-10 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-10 in the samples. A monoclonal antibody specific for human MMP-10 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and MMP-10 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for MMP-10 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-10 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
