Human MMP-8 ELISA Kit (A1492)

Human MMP-8 ELISA Kit

Human MMP-8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MMP-8 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human MMP-8 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-8 in the samples. A monoclonal antibody specific for human MMP-8 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MMP-8, and MMP-8 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-8 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

12.66 pg/ml

78.13 - 5000 pg/ml

This kit can assay both native and recombinant human MMP-8.

Human

This ELISA kit has shown no significant cross-reactivity.

97% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CLG1, Matrix metalloproteinase-8, Neutrophil collagenase, PMNL collagenase, PMNL-CL

Information online is representative. Always refer to the Product Manual inside the kit.