Human MMP-9 ELISA Kit (Small Sample Volume) (A351848)

Human MMP-9 ELISA Kit (Small Sample Volume)

Human MMP-9 ELISA Kit (Small Sample Volume) is a 3h 30m sandwich Enzyme-Linked Immunosorbent Assay (sELISA) optimized for small sample volumes requiring only 25 µL of sample, designed for the in vitro quantitative determination of human MMP-9 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Human MMP-9 ELISA Kit (Small Sample Volume) (A351848) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of human in serum, plasma, and other biological fluids. This assay is optimized for limited sample availability, requiring only 25 µL of sample per well. An antibody specific for MMP-9 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MMP-9 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with biotinylated anti-MMP-9 antibody, which binds the captured MMP-9 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of MMP-9 captured in each well. The concentration of MMP-9 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-Coated 96 Well Microplate, Reference Standard, Biotinylated Detection Antibody, Concentrated Biotinylated Detection Antibody, HRP Conjugate, Concentrated HRP Conjugate, Reference Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer (25X), TMB Substrate, Stop Solution, Plate Sealers, Product Manual.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

23.44 pg/mL

39-2500 pg/mL

3h 30m

Human

Specific for MMP-9 with no reported cross-reactivity with other analogs or variants.

Information online is representative. Always refer to the Product Manual inside the kit.

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9

Information online is representative. Always refer to the Product Manual inside the kit.