Human/Mouse/Rat NF-L ELISA Kit (A334822)

Human/Mouse/Rat NF-L ELISA Kit

Human/Mouse/Rat NF-L ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human, mouse, rat NF-L in serum, plasma, and cell culture supernatant.

Sandwich (quantitative)

Human/Mouse/Rat NF-L ELISA Kit (A334822) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human, mouse, rat NF-L in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for NF-L. Standards and test samples are added to the wells, allowing NF-L to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of NF-L captured in each well. The concentration of NF-L is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 35 mL Assay Diluent #11R, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well H-Microplate (12 x 8 well strips), and 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, and cell culture supernatant.

6.25 pg/ml - 400 pg/ml

1 hour

Human, Mouse, Rat

Cell culture supernatant: 110%; Serum: 101%; Plasma: 94%

Intra-assay: 3.6%, Inter-assay: 6.8%

The immunogens for the antibodies in this kit are recombinant NFL.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

This ELISA kit uses recombinant rabbit monoclonal antibodies.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

68 kDa neurofilament protein, 68kDa Neurofilament, NEFL, Neurofilament light polypeptide, Neurofilament triplet L protein, NF68, NFL

Information online is representative. Always refer to the Product Manual inside the kit.