Human NPPC ELISA Kit (A78535)

Human NPPC ELISA Kit

Competitive

This Human NPPC ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of NPPC in plasma, tissue homogenates and other biological fluids.

A microplate has been precoated with NPPC. Samples, standards and Anti-NPPC Antibody (Biotin) are added to the wells, where NPPC in the sample competes with the fixed NPPC to bind to the antibody. The wells are washed to remove unbound material and HRP-conjugated streptavidin is added. After incubation, the wells are washed again and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of NPPC in the sample and the intensity is measured at 450 nm.

Microplate

Colorimetric

< 37.5 pg/ml

62.5 - 4000 pg/ml

Human

95%

4°C for 6 months.

C-type natriuretic peptide, CNP2

Information online is representative. Always refer to the Product Manual inside the kit.