Human Osteopontin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Osteopontin in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Osteopontin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human OPN in the samples. A monoclonal antibody specific for human OPN has been immobilized onto microwells. Standard or samples are pipetted into the wells, and OPN present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for OPN is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPN bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
4.42 pg/ml
Product Range
125 - 8000 pg/ml
Specificity
This kit can assay both native and recombinant human OPN.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
99% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
BNSP, Bone sialoprotein 1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1, Urinary stone protein, Uropontin
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
