Human P-Selectin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human P-Selectin in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human P-Selectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sP-Selectin in the samples. A monoclonal antibody specific for human sP-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sP-Selectin, and sP-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sP-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
1.11 pg/ml
Product Range
78.13 - 5000 pg/ml
Specificity
This kit can assay both native and recombinant human P-Selectin.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
96% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
CD62 antigen-like family member P, GMP-140, GMRP, Granule membrane protein 140, GRMP, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, Platelet activation dependent granule-external membrane protein, SELP
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
