Human Perforin ELISA Kit (A341750)

Human Perforin ELISA Kit

Human Perforin ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human Perforin in biological samples.

Sandwich (quantitative)

Human Perforin ELISA Kit (A341750) is a sandwich ELISA designed for the sensitive and quantitative measurement of human Perforin in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Perforin. Standards and samples are added to the wells, allowing Perforin present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Perforin is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Perforin in the sample. The concentration of Perforin is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

40 pg/ml

62.5-2000 pg/ml

2h 45m

This product recognizesnatural human Perforin.

Human

This product shows no cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFalpha, IFN?, Fas, Fas L, IL-1b, IL-2.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

Cytolysin, Lymphocyte pore-forming protein, P1, Perforin-1, PFP, PRF1

Information online is representative. Always refer to the Product Manual inside the kit.