Human Peroxiredoxin 5 ELISA Kit (A330038)

Human Peroxiredoxin 5 ELISA Kit

Human Peroxiredoxin 5 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Peroxiredoxin 5 in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Human Peroxiredoxin 5 ELISA Kit (A330038) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Peroxiredoxin 5 in serum, plasma and cell culture supernates. An antibody specific for Peroxiredoxin 5 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Peroxiredoxin 5 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Peroxiredoxin 5 Antibody, which binds the captured Peroxiredoxin 5 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Peroxiredoxin 5 captured in each well. The concentration of Peroxiredoxin 5 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

0.097 ng/ml

0.39-25 ng/ml

4 hours

100µl

Human

Intra-assay CV: <10%, Inter-assay CV: <15%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-Peroxiredoxin 5 Antibody (A329736) as the capture antibody and Anti-Peroxiredoxin 5 Antibody (A329737) as the detection antibody.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, Peroxiredoxin V, Peroxiredoxin-5, mitochondrial, PLP, PRDX5, Prx-V

Information online is representative. Always refer to the Product Manual inside the kit.