Principle of Assay
Human SARS-CoV-2 Neutralization Antibody ELISA Kit (A327007) employs the competitive enzyme immunoassay technique for the quantitative measurement of human SARS-CoV-2 Neutralization Antibody in serum and plasma. The 96-well microtiter plate has been pre-coated with SARS-CoV-2 Neutralization Antibody antigen. During the incubation, SARS-CoV-2 Neutralization Antibody present in the samples or standards competes with the fixed amount of immobilized SARS-CoV-2 Neutralization Antibody for binding sites on the Biotinylated Anti-SARS-CoV-2 Neutralization Antibody Antibody. The more SARS-CoV-2 Neutralization Antibody present in a sample or standard, the less Biotinylated Anti-SARS-CoV-2 Neutralization Antibody Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-SARS-CoV-2 Neutralization Antibody Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of SARS-CoV-2 Neutralization Antibody present in each sample or standard. The concentration of SARS-CoV-2 Neutralization Antibody can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
