Human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM ELISA Kit (A319516)

Human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM ELISA Kit

Human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM ELISA Kit is a 3 hour indirect sandwich Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM in serum, plasma, tissue homogenates, and other biological fluids.

Indirect (quantitative)

Human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM ELISA Kit (A319516) is a 3 hour indirect sandwich Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added. This detection antibody binds the captured Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM to form an antigen-antibody-detection antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM captured in each well. The concentration of Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

0.781-50 ng/mL

3 hours

Human

Specific for Anti-SARS-CoV-2 Spike RBD (alpha B.1.1.7) IgM with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.