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Human SQSTM1 / p62 ELISA Kit (A311689)

Standard Curve - Human SQSTM1 / p62 ELISA Kit (A311689) - Antibodies.com
This product is discontinued

Human SQSTM1 / p62 ELISA Kit (A311689) has been discontinued and is no longer available.

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Name
Human SQSTM1 / p62 ELISA Kit
Description
Human SQSTM1 / p62 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human SQSTM1 / p62 in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human SQSTM1 / p62 ELISA Kit (A311689) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human SQSTM1 / p62 in An antibody specific for SQSTM1 / p62 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SQSTM1 / p62 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-SQSTM1 / p62 Antibody, which also binds the SQSTM1 / p62 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SQSTM1 / p62 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SQSTM1 / p62 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of SQSTM1 / p62 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of SQSTM1 / p62 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma or other biological fluids.
Sensitivity
0.023 ng/ml
Product Range
1-16 ng/ml
Assay Time
1h 30m
Reactivity
Human
Precision
Intra-assay CV: 5%, Inter-assay CV: < 10%
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
A170, DMRV, EBI 3 associated protein of 60 kDa, EBI 3 associated protein p60, EBI3 associated protein of 60 kDa, EBI3 associated protein p60, EBI3-associated protein of 60 kDa, EBIAP, FTDALS3, MGC127197, ORCA, OSF-6, Osi, OSIL, Oxidative stress induced like, p60, p62, p62B, Paget disease of bone 3, PDB 3, PDB3, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa, Phosphotyrosine independent ligand for the Lck SH2 domain p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, PKC-zeta-interacting protein, Protein kinase C-zeta-interacting protein, Sequestosome 1, Sequestosome-1, SQSTM 1, Sqstm1, SQSTM_HUMAN, STAP, STONE14, Ubiquitin binding protein p62, Ubiquitin-binding protein p62, ZIP, ZIP 3, ZIP3
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Kit Components
Item Quantity Storage
Pre-Coated 96 Well Microplate 12 x 8 Well Strips +4°C
Standard Solution 500µl +4°C
Standard Diluent 3ml +4°C
Biotinylated Detection Antibody 1ml +4°C
Streptavidin-HRP 6ml +4°C
Wash Buffer (25X) 20ml +4°C
Substrate Solution A 6ml +4°C
Substrate Solution B 6ml +4°C
Stop Solution 6ml +4°C
Plate Sealers 5 Adhesive Strips -
Foil Pouch 1 Zip-Sealed Pouch -
Required Materials
The following materials are not included in the kit, but are required to run this assay:
  • Microplate reader capable of measuring absorbance at 450nm ± 10nm.
  • Squirt bottle, multichannel pipette reservoir, or automated microplate washer.
  • Graph paper or computer software capable of generating logarithmic functions.
  • Test tubes and Eppendorf tubes to prepare standards and samples.
  • Multi- and single-channel pipettes and sterile pipette tips.
  • Deionized or distilled water.
  • Absorbent paper towels.
  • 37°C incubator.
Important Notes
  • Samples cannot be diluted for use with this kit. Owing to the material used to prepare the kit, sample matrix interference may falsely depress the specificity and accuracy of the assay.
  • Sample concentrations should be estimated before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their experiments.
  • Samples containing Sodium Azide (NaN3) cannot be used in this assay. NaN3 inhibits the activity of Horseradish Peroxidase (HRP) which is used in the detection step.
  • This ELISA kit is intended for the analysis of biological samples and has not been validated for purified/recombinant proteins, industrial, or commercial materials. Performance with such samples cannot be guaranteed, as differences in expression, sequence, tags, folding, post-translational modifications, purity, activity, or buffer composition may affect assay results. For further guidance, please contact our technical support team.

  • Scientific Validation DataValidation Data (1)

Standard Curve - Human SQSTM1 / p62 ELISA Kit (A311689) - Antibodies.comEnlarge Image

Standard Curve - Human SQSTM1 / p62 ELISA Kit (A311689)

Representative standard curve for Human SQSTM1 / p62 ELISA Kit (A311689).