Human TFPI ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the rapid in vitro quantitative determination of human TFPI in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human TFPI ELISA Kit (A351934) employs a rapid sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of human TFPI in serum, plasma, and other biological fluids. An antibody specific for TFPI has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection antibody working solution. During incubation, TFPI present in the sample binds to the immobilized capture antibody and forms a sandwich immune complex with the HRP-conjugated detection antibody. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TFPI captured in each well. The concentration of TFPI is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.
Linearity assessment of the Human TFPI ELISA Kit (A351934) across serial sample dilutions. Linearity was evaluated using Serum and EDTA Plasma samples serially diluted from 1:2 to 1:16. Recovery (%) was calculated relative to the expected Human TFPI concentration at each dilution. Data are presented as mean recovery values from 8 replicates (n = 8).
Quick workflow summary for 90 Minute ELISA Kits (Sandwich). Samples or standards (50 µL per well) are incubated in antibody pre-coated microplate wells together with an HRP-conjugated detection antibody, enabling rapid formation of a sandwich immune complex. Following incubation and wash steps to remove unbound components, TMB substrate is added for color development. The enzymatic reaction is terminated using stop solution prior to optical density measurement at 450 nm. This streamlined workflow enables fast, sensitive, and reliable quantitative analyte detection in biological samples.
Streamlined rapid sandwich ELISA workflow for fast and sensitive analyte quantification. This rapid 90-minute sandwich ELISA workflow enables sensitive and reliable quantitative detection of target analytes in biological samples using a simplified incubation and detection protocol. Target analytes are captured by immobilized antibodies and detected using specific detection antibodies to form sandwich immune complexes. Following substrate development, colorimetric signal intensity is measured and is directly proportional to analyte concentration within the sample. This optimized workflow supports high-performance ELISA analysis with reduced assay time and efficient sample processing.
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