Human TGF beta 1 ELISA Kit (A341704)

Human TGF beta 1 ELISA Kit

Human TGF beta 1 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human TGF beta 1 in biological samples.

Sandwich (quantitative)

Human TGF beta 1 ELISA Kit (A341704) is a sandwich ELISA designed for the sensitive and quantitative measurement of human TGF beta 1 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for TGF beta 1. Standards and samples are added to the wells, allowing TGF beta 1 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for TGF beta 1 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of TGF beta 1 in the sample. The concentration of TGF beta 1 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

8.6 pg/ml

62.5-2000 pg/ml

4h 30m

100 µl

This product recognizes both natural and recombinant human TGF-beta1

Human

Cross reactivity with sheep and pig TGF-beta1.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C

TGFB, TGFB1, Transforming growth factor beta-1 proprotein

Information online is representative. Always refer to the Product Manual inside the kit.