Human Thrombospondin-1 ELISA Kit (A1524)

Human Thrombospondin-1 ELISA Kit

Human Thrombospondin-1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Thrombospondin-1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Thrombospondin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human THBS-1 in the samples. A monoclonal antibody specific for human THBS-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for THBS-1, and THBS-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of THBS-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

26.39 pg/ml

0.78 - 50 ng/ml

This kit can assay both native and recombinant human THBS-1.

Human

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

Glycoprotein G, THBS1, TSP, TSP1

Information online is representative. Always refer to the Product Manual inside the kit.