Principle of Assay
Human Tie1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Tie1 in the samples. A monoclonal antibody specific for human Tie1 has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for Tie1 are pipetted into the wells and Tie1 present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Tie1 bound in the initial step. The color development is stopped and the intensity of the color is measured.