Human TIM-1 ELISA Kit (A1526)

Human TIM-1 ELISA Kit

Human TIM-1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TIM-1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human TIM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TIM-1 in the samples. A monoclonal antibody specific for human TIM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIM-1, and TIM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human TIM-1.

Human

This ELISA kit has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CD365, HAVcr-1, HAVCR1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, KIM1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, TIM, TIM1, TIMD-1, TIMD1

Information online is representative. Always refer to the Product Manual inside the kit.