Human TIMP-1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TIMP-1 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human TIMP-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TIMP-1 in the samples. A monoclonal antibody specific for human TIMP-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIMP-1, and TIMP-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIMP-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
3.94 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both native and recombinant human TIMP-1.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, TIMP, Tissue inhibitor of metalloproteinases 1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
