Principle of Assay
Human TMEM127 ELISA Kit (A75076) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TMEM127 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for TMEM127. Standards and samples are added to the wells, where TMEM127 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for TMEM127 is added to the wells. The detection antibody binds the captured TMEM127 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TMEM127 captured in each well. The concentration of TMEM127 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
