Human TNF alpha ELISA Kit (A334799)

Human TNF alpha ELISA Kit

Human TNF alpha ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TNF alpha in serum, plasma, and cell culture supernatant.

Sandwich (quantitative)

Human TNF alpha ELISA Kit (A334799) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human TNF alpha in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for TNF alpha. Standards and test samples are added to the wells, allowing TNF alpha to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 50 mL Assay Diluent #1, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well A-Microplate (12 x 8 well strips), and 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, and cell culture supernatant.

21.9 pg/ml - 1404.6 pg/ml

1 hour

Human

Cell culture supernatant: 95%; Serum: 103%; Plasma: 108%

Intra-assay: 6.8%, Inter-assay: 8.9%

The immunogens for the antibodies in this kit are recombinant TNF- alpha.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

This ELISA kit uses recombinant rabbit monoclonal antibodies.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2

Information online is representative. Always refer to the Product Manual inside the kit.