Human TNFR1 ELISA Kit (A330047)

Human TNFR1 ELISA Kit

Human TNFR1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TNFR1 in serum, plasma and cell culture supernates.

Sandwich (quantitative)

Human TNFR1 ELISA Kit (A330047) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TNFR1 in serum, plasma and cell culture supernates. An antibody specific for TNFR1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNFR1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TNFR1 Antibody, which binds the captured TNFR1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNFR1 captured in each well. The concentration of TNFR1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

1.82 pg/ml

7.81-500 pg/ml

4 hours

100µl

Human

Intra-assay CV: <4.1%, Inter-assay CV: <7.1%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-TNFR1 Antibody (A329915) as the capture antibody and Anti-TNFR1 Antibody (A329916) as the detection antibody.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

CD120a, p55, p60, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFRSF1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I

Information online is representative. Always refer to the Product Manual inside the kit.