Human Transferrin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Transferrin in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Transferrin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TF in the samples. A monoclonal antibody specific for human TF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for TF is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
2.82 pg/ml
Product Range
937.50 - 60000 pg/ml
Specificity
This kit can assay both native and recombinant human TF.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.