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Human Treponema pallidum Antibody ELISA Kit (A79862)

$605

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-11 business days.
Name
Human Treponema pallidum Antibody ELISA Kit
Description
Human Treponema pallidum Antibody ELISA Kit is a 3 hour qualitative Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro qualitative determination of human Treponema pallidum Antibody in serum, plasma, tissue homogenates, and other biological fluids.
Assay Type
Indirect (qualitative)
Principle of Assay
Human Treponema pallidum Antibody ELISA Kit (A79862) is a 3 hour qualitative Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro qualitative detection of human Treponema pallidum Antibody in serum, plasma, tissue homogenates, and other biological fluids. Samples are added to a 96-well microtiter plate pre-coated with assay-specific binding reagents. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection reagent is added. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The presence or absence of colour indicates the presence or absence of Treponema pallidum Antibody in the sample.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Assay Time
3 hours
Reactivity
Human
Cross Reactivity
Demonstrates specificity for Treponema pallidum antibodies with no reported cross-reactivity to related analogs.
General Notes
This qualitative ELISA provides only a positive or negative result and does not measure or quantify the concentration of the analyte in the sample.
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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