Principle of Assay
Human VEGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VEGF in the samples. A monoclonal antibody specific for human VEGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for VEGF, and VEGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
