Human VCAM1 ELISA Kit (A1521)

Human VCAM1 ELISA Kit

Human VCAM1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human VCAM1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human sVCAM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sVCAM-1 in the samples. A monoclonal antibody specific for human sVCAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sVCAM-1, and sVCAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sVCAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.65 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant human sVCAM-1.

Human

This ELISA kit has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CD106, INCAM-100, V-CAM 1, Vascular cell adhesion protein 1, VCAM-1

Information online is representative. Always refer to the Product Manual inside the kit.