Human VEGF Receptor 2 ELISA Kit (A1537)

Human VEGF Receptor 2 ELISA Kit

Human VEGF Receptor 2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human VEGF Receptor 2 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human VEGF R2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VEGF R2 in the samples. A monoclonal antibody specific for human VEGF R2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for VEGF R2, and VEGF R2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF R2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.52 pg/ml

62.50 - 4000 pg/ml

This kit can assay both native and recombinant human VEGF R2.

Human

This ELISA kit has shown no significant cross-reactivity.

94% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CD309, Fetal liver kinase 1, FLK-1, FLK1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, Vascular endothelial growth factor receptor 2, VEGFR-2, VEGFR2

Information online is representative. Always refer to the Product Manual inside the kit.