Human Vitamin D Binding Protein ELISA Kit (A1540)

Human Vitamin D Binding Protein ELISA Kit

Human Vitamin D Binding Protein ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Vitamin D Binding Protein in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Vitamin D BP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Vitamin D BP in the samples. A monoclonal antibody specific for human Vitamin D BP has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Vitamin D BP, and Vitamin D BP present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Vitamin D BP bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

40.15 pg/ml

78.13 - 5000 pg/ml

This kit can assay both native and recombinant human Vitamin D BP.

Human

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

DBP, DBP-maf, GC, Gc protein-derived macrophage activating factor, Gc-globulin, Gc-MAF, GcMAF, Group-specific component, VDB, Vitamin D-binding protein, Vitamin D-binding protein-macrophage activating factor

Information online is representative. Always refer to the Product Manual inside the kit.