Principle of Assay
Human Vitronectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VTN in the samples. A monoclonal antibody specific for human VTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for VTN is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of VTN bound in the initial step. The color development is stopped and the intensity of the color is measured.