Human vWF ELISA Kit (A1542)

Human vWF ELISA Kit

Human vWF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human vWF in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human vWF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human vWF in the samples. A monoclonal antibody specific for human vWF has been immobilized onto microwells. Standard or samples are pipetted into the wells, and vWF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for vWF is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of vWF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.47 pg/ml

78.13 - 5000 pg/ml

This kit can assay both native and recombinant human vWF.

Human

This ELISA kit has shown no significant cross-reactivity.

101% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

F8VWF, von Willebrand factor

Information online is representative. Always refer to the Product Manual inside the kit.