Human ZnT8 ELISA Kit is an indirect elisa Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human ZnT8 in
Assay Type
Indirect (quantitative)
Principle of Assay
Human ZnT8 ELISA Kit (A335032) is an Indirect ELISA for the quantitative measurement of human ZnT8 in . The 96-well microtiter plate has been pre-coated with ZnT8 antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of ZnT8 sample captured on the plate. The concentration of ZnT8 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sensitivity
0.938 ng/ml
Product Range
1.563-100 ng/ml
Assay Time
4 hours
Reactivity
Human
Recovery
Sample Type
n
Range
Average
Serum
10
88-101
95
EDTA plasma
10
85-98
91
Heparin plasma
10
87-105
95
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
85-101%
87-100%
87-96%
EDTA plasma
10
90-105%
85-99%
87-100%
Heparin plasma
10
85-100%
91-99%
85-92%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
Proton-coupled zinc antiporter SLC30A8, SLC30A8, Solute carrier family 30 member 8, Zinc transporter 8, ZnT-8
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
