IL-2R beta Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of IL2 Receptor beta levels in adherent and suspension cells.
Assay Type
Cell-based (qualitative)
Principle of Assay
This IL-2R beta Cell Based ELISA Kit allows for the detection of IL-2R beta and the effects that certain stimulation conditions have on IL-2R beta expression in different cell lines. Qualitative determination of IL-2R beta concentration is achieved by an indirect ELISA format. In essence, the IL-2R beta is captured by Anti-IL-2R beta Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IL-2R beta Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Western Blot - IL-2R beta Cell Based ELISA Kit (A102891)
The mouse monoclonal antibody to GAPDH is used as a positive control inIL-2R beta Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Proteins predicted to interact with IL2 Receptor beta
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
