LHR2A Cell Based ELISA Kit (A103687)

LHR2A Cell Based ELISA Kit

Cell-based (qualitative)

This LHR2A Cell Based ELISA Kit allows for the detection of LHR2A and the effects that certain stimulation conditions have on LHR2A expression in different cell lines. Qualitative determination of LHR2A concentration is achieved by an indirect ELISA format. In essence, the LHR2A is captured by Anti-LHR2A Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this LHR2A Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 6 months.

BCSC 1, BCSC-1, BCSC1, Breast cancer suppressor candidate 1, LOH11CR2A, Loss of heterozygosity 11 chromosomal region 2 gene A, Loss of heterozygosity 11 chromosomal region 2 gene A protein, Ortholog of mouse AW551984, VMA5A_HUMAN, von Willebrand factor A domain containing 5A, von Willebrand factor A domain-containing protein 5A, Vwa5a

Information online is representative. Always refer to the Product Manual inside the kit.