Lyme Disease Borrelia burgdorferi sensu lato IgG ELISA Kit (A341253)

Lyme Disease Borrelia burgdorferi sensu lato IgG ELISA Kit

Lyme Disease Borrelia burgdorferi sensu lato IgG ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) designed for the in vitro quantitative determination of IgG antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma.

Indirect ELISA

Lyme Disease Borrelia burgdorferi sensu lato IgG ELISA Kit (A341251) employs an indirect ELISA for the quantitative measurement of IgG antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma. The 96-well microtiter plate is pre-coated with Borrelia antigens. Samples and standards are added to the wells and incubated, allowing specific IgG antibodies to bind to the immobilized antigens. After washing to remove unbound material, a biotinylated detection antibody is added, followed by HRP-streptavidin. Following a further wash step, TMB substrate is added to visualize the enzymatic reaction. HRP catalyzes the conversion of TMB to a blue product, which turns yellow upon addition of acidic stop solution. The intensity of the yellow colour is proportional to the amount of Borrelia-specific IgG bound to the plate. Absorbance is measured at 450 nm using a microplate reader, and antibody concentrations are determined from a standard curve.

This ELISA kit includes a 96-well microplate pre-coated with proprietary Epitogen Lyme antigens and supplied ready for use. The kit also contains a peroxidase-labelled anti-human IgG antibody, 10× wash buffer, secondary diluent, TMB substrate and a protective plate seal.

Pre-coated Microplate (12 x 8 well strips)

HRP Colorimetric

Serum and plasma.

The assay demonstrates 100 percent sensitivity in late-stage Lyme disease, including Lyme neuroborreliosis and Lyme carditis, and shows greater than 87.8 percent sensitivity in acute Lyme disease within six weeks of infection.

1h 40m

B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii. B. spielmanii. B. bissetiae, B. bavariensis and B. valaisiana

This kit uses an immunogen composed of a mixture of 120 linear immunodominant epitopes, 4 flagellin subunits, 12 full-length VlsE proteins, and 8 full-length OspC antigens to detect IgG antibodies associated with established or past Borrelia infection.

IgG

Shipped at 4°C. Store at 4°C.

Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1, Immunoglobulin heavy constant gamma 1

Information online is representative. Always refer to the Product Manual inside the kit.