Monkey ACTH ELISA Kit (A327059)

Monkey ACTH ELISA Kit

Monkey ACTH ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey ACTH in serum, plasma, and other biological fluids.

Competitive (quantitative)

Monkey ACTH ELISA Kit (A327059) employs the competitive enzyme immunoassay technique for the quantitative measurement of monkey ACTH in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with ACTH antigen. During the incubation, ACTH present in the samples or standards competes with the fixed amount of immobilized ACTH for binding sites on the Biotinylated Anti-ACTH Antibody. The more ACTH present in a sample or standard, the less Biotinylated Anti-ACTH Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-ACTH Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of ACTH present in each sample or standard. The concentration of ACTH can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

9.38 pg/ml

15.63-1000 pg/ml

2 hours

Monkey

No significant cross-reactivity or interference between monkey ACTH and analogues was observed.

Intra-assay CV: 4.7%, Inter-assay CV: 4.79%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, alpha-MSH, alphaMSH, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, beta-MSH, CLIP, Corticotropin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, gamma-MSH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, LPH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, MSH, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin, Proopiomelanocortin preproprotein, Tetracosactide

Information online is representative. Always refer to the Product Manual inside the kit.